研究業績

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学術論文

• Chow-Lih Yew, Takashi Tsuchimatsu, Rie Shimizu-Inatsugi, Shinsuke Yasuda, Masaomi Hatakeyama, Hiroyuki Kakui, Takuma Ohta, Keita Suwabe, Masao Watanabe, Seiji Takayama & Kentaro K. Shimizu (2023)Dominance in self-compatibility between subgenomes of allopolyploid Arabidopsis kamchatica shown by transgenic restoration of self-incompatibility, Nature Communications 14, 7618 [link][日本語プレスリリース]

• Shuhei Nasuda, Kazuki Murata, Hiroyuki Kakui, Koichi Yamamori, Takanori Yoshikawa (2023) Molecular cytological identification of the gametocidal chromosome discovered by Endo and Tsunewaki (1975) in an alloplasmic wheat, Cytologia 88(3) 167-168 [link] *表紙カバーに選出

• Hiroyuki Kakui, Tokuko Ujino-Ihara, et al. (2023) A single-nucleotide substitution of CjTKPR1 determines pollen production in the gymnosperm plant Cryptomeria japonica, PNAS Nexus 2(8) pgad236 [link][日本語プレスリリース]

• Yoshinari Moriguchi, Ryunosuke Saito, Saneyoshi Ueno, Yoichi Hasegawa, Hiroyuki Kakui, Asako Matsumoto (2022) Localization of TWISTED NEEDLES Locus on Linkage Map of Japanese Cedar (Cryptomeria japonica D. Don), forests 13, 1524 [link]

• 角井宏行, 釣崎 恵里子, 柴田 嶺, 佐々 英徳, 森口 喜成 (2022) ハイスループットな花粉数計測法の開発と、 スギ1 雄花あたりの花粉数に影響する要因についての研究, 森林遺伝育種 11(2) 97-103

• 角井宏行, 山﨑美紗子, 清水健太郎 (2022) 電気泳動でDNAの1塩基差を迅速・簡便に識別する遺伝子型判別法：PRIMA, 実験医学 40(4) 601-608 [link]

• Hiroyuki Kakui, Takashi Tsuchimatsu, Misako Yamazaki, Masaomi Hatakeyama, Kentaro K Shimizu (2022) Pollen number and ribosome gene expression altered in a genome-editing mutant of REDUCED POLLEN NUMBER1 gene, Frontiers in Plant Science 12(768584) [link]

• 角井宏行 (2021) 効率的な遺伝子型判別法と花粉数計測法が加速する花粉数研究, アグリバイオ 5(14) 51-53 [link]

• Hiroyuki Kakui, Misako Yamazaki, Kentaro K. Shimizu (2021) PRIMA: a rapid and cost-effective genotyping method to detect single-nucleotide differences using probe-induced heteroduplexes, Scientific Reports 11(20741) [link][日本語プレスリリース]

• Hiroyuki Kakui, Eriko Tsurisaki, Rei Shibata, Yoshinari Moriguchi (2021) Factors affecting the number of pollen grains per male strobilus in Japanese Cedar (Cryptomeria japonica), Plants 10(5) 856 [link]

• 角井宏行, 土松隆志, 山﨑美紗子, 清水健太郎 (2021) 花粉数を制御する遺伝子の発見, バイオサイエンスとインダストリー 79(1) 32-33

• Hiroyuki Kakui, Eriko Tsurisaki, Hidenori Sassa, Yoshinari Moriguchi (2020) An improved pollen number counting method using a cell counter and mesh columns, Plant Methods 16(124) 1-13 [link]

• 角井宏行, 土松隆志, 山﨑美紗子, 清水健太郎 (2020) シロイヌナズナの花粉数を制御する遺伝子 – 少ない花粉と生育メリットを両立するカギ, academist Journal, pp. 14530 [link]

• Takashi Tsuchimatsu*, Hiroyuki Kakui*, Misako Yamazaki, Cindy Marona, Hiroki Tsutsui, Afif Hedhly, Dazhe Meng, Yutaka Sato, Thomas Städler, Ueli Grossniklaus, Masahiro M Kanaoka, Michael Lenhard, Magnus Nordborg, Kentaro K Shimizu (2020) Adaptive reduction of male gamete number in the selfing plant Arabidopsis thaliana, Nature Communications 11(1) 2885 *共同第一著者 [link][日本語プレスリリース]

• Chow-Lih Yew, Hiroyuki Kakui, Kentaro K. Shimizu (2018) Agrobacterium-mediated floral dip transformation of the model polyploid species Arabidopsis kamchatica, Journal of Plant Research 131(2) 349-358 [link]

• 高橋有, 山内卓樹, 角井宏行, 寺内良平, 大柳一, 小宮怜奈, 宮武宏治, 内藤健 (2018) 次世代の遺伝学と育種, 育種学研究 15(3) 115-121 [link]

• Mai F. Minamikawa, Daisuke Fujii, Hiroyuki Kakui, Nobuhiro Kotoda, Hidenori Sassa (2013) Identification of an S-RNase binding protein1 (SBP1) homolog of apple (Malus×domestica), Plant Biotechnology 30(2) 119-123 [link]

• Sanhong Wang, Hiroyuki Kakui, Shinji Kikuchi, Takato Koba, Hidenori Sassa (2012) Interhaplotypic heterogeneity and heterochromatic features may contribute to recombination suppression at the S locus in apple (Malusdomestica), Journal of Experimental Botany 63(13) 4983-4990 [link]

• Hidenori Sassa, Sanhong Wang, Minamikawa Minamikawa, Hiroyuki Kakui, Shinji Kikuchi, Takato Koba (2012) Structural Features of the S Locus of Apple, Acta Horticulturae 967 89-94 [link]

• Hiroyuki KakuiHiroyuki Kakui, Masaki Kato, Koichiro Ushijima, Miyoko Kitaguchi, Shu Kato, Hidenori Sassa (2011) Sequence divergence and loss-of-function phenotypes of S locus F-box brothers genes are consistent with non-self recognition by multiple pollen determinants in self-incompatibility of Japanese pear (Pyrus pyrifolia), The Plant Journal 68(6) 1028-1038 [link]

• Mai Hayashi, Aya Ujiie, Hiroaki Serizawa, Hidenori Sassa, Hiroyuki Kakui, Tetsuya Oda, Takato Koba (2011) Development of SCAR and CAPS markers linked to a recessive male sterility gene in lettuce (Lactuca sativa L.), Euphytica 180(3) 429-436 [link]

• Mai Minamikawa, Hiroyuki Kakui, Sanhong Wang, Nobuhiro Kotoda, Shinji Kikuchi, Takato Koba, Hidenori Sassa (2010) Apple S locus region represents a large cluster of related, polymorphic and pollen-specific F-box genes, Plant Molecular Biology 74(1-2) 143-154 [link]

• Hidenori Sassa, Hiroyuki Kakui, Mai Minamikawa (2010) Pollen-expressed F-box gene family and mechanism of S-RNase-based gametophytic self-incompatibility (GSI) in Rosaceae, Sexual Plant Reproduction 23(1) 39-43 [link]

• Hoytaek Kim, Hoytaek Kim, Hiroyuki Kakui, Nobuhiro Kotoda, Nobuhiro Kotoda, Yutaka Hirata, Takato Koba, Hidenori Sassa (2009) Determination of partial genomic sequences and development of a CAPS system of the S-RNase gene for the identification of 22 S haplotypes of apple (Malus × domestica Borkh.), Molecular Breeding 23(3) 463-472 [link]

• Hiroyuki Kakui, Takeshi Tsuzuki, Takato Koba, Hidenori Sassa (2007) Polymorphism of SFBB-gamma and its use for S genotyping in Japanese pear (Pyrus pyrifolia), Plant Cell Reports 26(9) 1619-1625 [link]

• Hoytaek Kim, Hiroyuki Kakui, Takato Koba, Yutaka Hirata, Hidenori Sassa (2007) Cloning of a new S-RNase and development of a PCR-RFLP system for the determination of the S-genotypes of Japanese pear, Breeding Science 57(2) 159-164 [link]

• Hidenori Sassa*, Hiroyuki Kakui*, Mayu Miyamoto, Yusuke Suzuki, Toshio Hanada, Koichiro Ushijima, Makoto Kusaba, Hisashi Hirano, Takato Koba (2007) S locus F-Box brothers: Multiple and pollen-specific F-box genes with S haplotype-specific polymorphisms in apple and Japanese pear, Genetics 175(4) 1869-1881 *共同第一著者 [link]

総説・解説記事など

• Hiroyuki Kakui, Misako Yamazaki, Naoto-Benjamin Hamaya, Kentaro K. Shimizu (2022)Pollen Grain Counting Using a Cell Counter, Pollen and Pollen Tube Biology In Methods in Molecular Biology series No. 2160  p1-11 [link]

• 角井宏行, 山﨑美沙子, 清水健太郎, 曽我部有司 (2022) 1塩基の変異を迅速かつ簡便に検出する方法 -MultiNAを活用したゲノム編集の検出法“PRIMA”-, 島津製作所アプリケーションノート, No.79 [link]

• 角井宏行, 岩田洋佳 (2022)「先生方、突撃させてください!」新任の先生にインタビューしてみた, 東京大学農学生命科学研究科 応用生物学専修&生産・環境生物学専攻 学生広報誌

特許

• Hiroyuki Kakui, Misako Yamazaki, Kentaro K Shimizu (2020) 特願PCT/EP2020/072434 Probe induced heteroduplex mobility assay (PRIMA) [link]

受賞

• 日本分子生物学会 第45回年会優秀発表賞, 2022年12月
• 日本育種学会 第141回講演会優秀発表賞, 2022年5月
• 日本育種学会 第140回講演会優秀発表賞, 2021年11月
• 第一回超分野植物科学研究会 最優秀ポスター賞, 2021年6月
• 3rd International Plant Sciences Workshop, “Plant development and evolution: from molecules to Ecosystems” 最優秀ポスター賞, 2018年6月
• 日本育種学会 第128回講演会優秀発表賞, 2016年2月
• 日本育種学会 第118回講演会優秀発表賞, 2010年11月
• 平成20年度 千葉大学自然科学研究科長表彰, 2009年3月